Skill

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Skill

Our Skill to study the metabolism in the gonad

In the Sensor team, we use in vitro cell exposure strategies (lineage, primary cells), as well as animal tissues / models (poultry, mouse, human)

- To study the different signaling pathways, we stimulate or inhibit them using Cre / lox, Crispr / Cas9, shRNA or pharmacological inhibitors on cultures of lines (granulosa, Sertoli, germinal cells). .), primary cell cultures (germ cells, ovarian cells, testicular cells, embryonic cells), as well as dynamic cultures with a circulating medium

culture cellulaire

- We express some vectors by lentiviral approaches

cell infection

(granulosa cells expressing GFP)

- We measure the expression of certain proteins using western blot, qPCR, immunolabeling

Etude de l'expression par immunohistochimie

- The field tests are validated by the ethics committees and the analyzes are carried out taking into account the best animal welfare (ex: measurement of the body mass by medical imaging), analysis with the minimum of blood test

Imagerie Scanner et folliculogenese

(left: analysis of the body mass by medical imaging, imaging CIRE, right: taking of the ovarian follicles)

Modification date : 02 August 2023 | Publication date : 10 December 2018 | Redactor : PF