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The BioForA unit has been working for many years on the improvement and conservation of several forest species. To this end, we have developed DNA extraction methods and molecular tools to characterize the diversity of these species.
These methods and tools can now be used routinely for traceability, detection of hybrids, clones, and pairings.

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Skills and tools for genotyping

• Extraction of DNA from different tissues (buds, leaves, needles, cambium, wood ...).
• Molecular marking tools available:

Example of applications

• traceability of poplar and cherry cultivars (we have access to reference clones).
• Detection of hybrids in larch, poplar.
• Quantification of clonality in poplars.
• Evaluation of the match (all species listed above).
• Determination of species in oaks
• Gene flow and monitoring of reproduction in the wild and in seed orchards

Equipments :

• Thermocyclers
• 8 capillary sequencer

Staff involved :

Bénédicte Le Guerroué, Vanina Benoit, Corinne Buret

Contact

vanina.benoit[at]inrae.fr

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Références

Philippe, G., Buret, C., Matz, S., Pâques, L. (2016). Composition of hybrid larch (Larix × eurolepis Henry) forest reproductive materials: How much does hybrid percentage affect stand performance? New Forests, 47 (4), 541–564. DOI : 10.1007/s11056-016-9530-z

Nardin, M., Musch, B., Rousselle, Y., Guérin, V., Sanchez, L., Rossi, J.-P., Gerber, S., Marin, S., Pâques, L. E., Rozenberg, P. (2015). Genetic differentiation of European larch along an altitudinal gradient in the French Alps. Annals of Forest Science, 72 (5), 517-527. DOI : 10.1007/s13595-015-0483-8

Dowkiw, A., Chenault, N., Guérin, V., Borel, C., Bastien, C., Villar, M. (2014). Post-pollination paternal reproductive success in Populus nigra: a male affair. Tree Genetics and Genomes, 10 (3), 565-572. DOI : 10.1007/s11295-014-0704-6

Oddou-Muratorio, S., Houot, M.L., Demesure-Musch, B., Austerlitz, F. (2003). Pollen flow in the wildservie tree, Sorbus torminalis (L.) Crantz. 1. Evaluating the paternity analysis procedure in continuous populations. Molecular Ecology, 12, 3427-3439.           

Oddou-Muratorio, S., Aligon, C., Decroocq, S., Plomion, C., Lamant, T., Mush-Demesure, B. (2001). Microsatellite primers for Sorbus torminalis and related species. Molecular Ecology Notes, 1, 297-299.

Stoeckel, S., Klein, E.K., Oddou-Muratorio, S., Musch, B., Mariette, S. (2012). Microevolution Of S-Allele Frequencies In Wild Cherry Populations: Respective Impacts Of Negative Frequency Dependent Selection And Genetic Drift. Evolution, 66 (2), 486-504. , DOI : 10.1111/j.1558-5646.2011.01457.x